BT3006 Biosensors Syllabus:

BT3006 Biosensors Syllabus – Anna University Regulation 2021

UNIT I FUNDAMENTALS OF BIOSENSOR

Biosensors as functional analogs of chemoreceptors, structure and function of transducers, qualitative and quantitative sensors, sensor parameters, transduction methods-optical, calorimetric, electrochemical and piezoelectric sensors Supports and support modifications-synthetic polymers,carbon material supports, metal supports, bifunctional crosslinkers.

UNIT II METABOLIC SENSORS

Methods of enzyme immobilization-adsorption, gel entrapment, covalent coupling, crosslinking immobilization effects in biosensors, characterisation of immobilized enzymes in biosensors,
effectiveness factor, enzyme loading test, Metabolic sensors-glucose, ascorbic acid, lactate sensors, determination of alcohols, sensors for phenols and amines, coupled enzyme reactors, sequence electrodes for nucleic acid , enzyme sensor for inhibitors.

UNIT III AFFINITY SENSORS AND REAGENTLESS SENSORS

Affinity sensors based on small ligands, immunosensors, immunoassay-RIA, ELISA and TELISA, piezoelectric immunosensors, optical immunosensors, electrochemical immunoassay,
Biocompatibility of sensors, biomimetic sensors, bioconjugated silica nanoparticles for bioanalysis.

UNIT IV NOVEL BIOSENSORS

Surface dielectric enhancement- gold nanoparticles enhanced surface plasmon resonance,magnetic biosensors and biochips, quantum dot based biosensors, DNA and protein conformational changes, optical and magnetic sensors, micro and nanocantilevers, electrochemical QCM,MEMS,PCR microchamber array chip system,Detection of target DNA on a single chip.

UNIT V APPLICATIONS OF BIOSENSORS

Biosensors and diabetes management, Microfabricated biosensors and point-of-care diagnostics systems, Noninvasive biosensors in clinical analysis; Surface plasmon resonance and evanescent wave biosensors, Biosensor in cancer and HIV early diagnosis.

TOTAL 45 PERIODS

COURSE OUTCOMES

Upon completion of this course, students will be able to
 Describe how bio specific interaction is used for various applications.
 Compare different techniques with emphasis on selectivity and sensitivity.
 Demonstrate knowledge of the general principles of sampling and manipulation of data generated by biosensors.
 Apply the knowledge to identify the various types of analytical methods.
 Design a system component or process to meet desired needs within realistic constraints.
 Recognize different types of transducers, and their application in biosensor design.

TEXT BOOKS:

1. Frieder Schelfer and Florian Schubert Biosensors Elsevier Science Publications 1992 2.
2. Challa Kumar Nanomaterials for Biosensors Wiley-VCH Verlag GMBH, Germany 2007.
3. Floriner-Gabriel Banica Chemical sensors and Biosensors-Fundamentals and Applications, John-Wiley & Sons Ltd, 2012.

REFERENCE

1. P. N. Bartlett (Ed.) Bioelectrochemistry- Fundamentals, Experimental techniques and applications, John Wiley & Sons, England 2008.
2. Nalwa (Ed.) Encyclopedia of Nanoscience and Nanotechnology 1 Vol. 5, 2004.