BT3561 Immunology Laboratory Syllabus:

BT3561 Immunology Laboratory Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES:

 To give practical training in the functioning of immune system.
 To give laboratory training in different immunological and immunotechnological techniques.

EXPERIMENTS

1. Animal Handling – Immunization – Bleeding techniques by Virtual methods
2. Identification of immune cells in a blood smear and Identification of blood group
2. Isolation of serum and plasma
3. Testing for typhoid antigens by Widal test
5. Immunodiffusion – SRID and Ouchterlony Double Diffusion
6. Immunoelectrophoresis – Classical, Counter Current and Rocket immunoelectrophoresis
7. Enzyme Linked ImmunoSorbent Assay (ELISA) – Types
8. Isolation of peripheral blood mononuclear cells
9. Isolation of monocytes from blood
10. Lymphocyte migration assay
11. Plaque forming cell assay
12. Identification of T cells by T-cell rossetting using sheep RBC.

TOTAL: 45 PERIODS

COURSE OUTCOMES:

 The students would be aware of immune system cells and tissues.
 The students would have knowledge on immunological /clinical tests.
 The students would be able to isolate lymphocytes and monocytes.
 The students would be able to identify various immune system cells.
 The students would become familiar with the techniques involved in antigen-antibody reaction
 The students will be able to identify the cellular and molecular basis of immune responsiveness

REFERENCES

1. Roitt I, Male, Brostoff. Immunology, Mosby Publ., 2002.
2. Kuby J, Immunology, WH Freeman & Co., 2000.
3. Ashim K. Chakravarthy, Immunology, TataMcGraw-Hill, 1998.