BT3751 Downstream Processing Syllabus:

BT3751 Downstream Processing Syllabus – Anna University Regulation 2021

OBJECTIVES:

To enable the students to
 Understand the methods to obtain pure proteins, enzymes and in general about product development R & D
 Have depth knowledge and hands on experience with on Downstream processes required in multi-factorial manufacturing environment in a structured and logical fashion

UNIT I INTRODUCTION

Introduction to downstream processing, principles, characteristics of bio-molecules and bioprocesses. Cell disruption for product release – mechanical, enzymatic and chemical methods.
Pre treatment and stabilisation of bio-products.

UNIT II PHYSICAL METHODS OF SEPARATION

Unit operations for solid-liquid separation – filtration and centrifugation.

UNIT III ISOLATION OF PRODUCTS

Adsorption, liquid-liquid extraction, aqueous two-phase extraction, membrane separation – ultrafiltration and reverse osmosis, dialysis, precipitation of proteins by different methods.

UNIT IV PRODUCT PURIFICATION

Chromatography – principles, instruments and practice, adsorption, reverse phase, ion exchange, size exclusion, hydrophobic interaction, bio-affinity and pseudo affinity chromatographic
techniques.

UNIT V FINAL PRODUCT FORMULATION AND FINISHING OPERATIONS

Crystallization, drying and lyophilization in final product formulation

TOTAL: 45 PERIODS

OUTCOMES:

Upon success completion of this course, the students will be able to:
 Define the fundamentals of downstream processing for product recovery
 Understand the requirements for successful operations of downstream processing
 Describe the components of downstream equipment and explain the purpose of each
 Apply principles of various unit operations used in downstream processing and enhance problem solving techniques

TEXT BOOKS:

1. Belter, P.A., E.L. Cussler and Wei-Houhu “Bioseparations – Downstream Processing for Biotechnology”, John Wiley, 1988.
2. Nooralabettu Krishna Prasad., Downstream Process Technology: A New Horizon in Biotechnology, Prentice Hall India,2010.
3. Sivasankar, B. “Bioseparations: Principles and Techniques”. PHI, 2006.

REFERENCES:

1. Raja Ghosh “Principles of Bioseparations Engineering”. World Scientific, 2006
2. Michael R.Ladisch Bioseparations Engineering: Principles, practice and Economics, WileyInterscience,1st Edition,2001.
3. Product Recovery in Bioprocess Technology”. (BIOTOL – Biotechnology by Open Learning Series). Butterworth – Heinmann / Elsevier, 2004.