BT3452 Industrial Enzymology Syllabus:

BT3452 Industrial Enzymology Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES:

To enable the students
 To learn enzyme reactions and its characteristics along with the production and purification process
 To attain a basic knowledge concerning biotransformation reactions with the usage of enzymes

UNIT I INTRODUCTION TO ENZYMES

Classification of enzymes. Mechanisms of enzyme action; concept of active site and energetics of enzyme substrate complex formation; specificity of enzyme action; principles of catalysis – collision theory, transition state theory; role of entropy in catalysis.

UNIT II KINETICS OF ENZYME ACTION

Kinetics of single substrate reactions; estimation of Michelis – Menten parameters, multisubstrate reactions – mechanisms and kinetics; turnover number; types of inhibition & models –substrate, product. Allosteric regulation of enzymes, Monod Changeux Wyman model, pH and temperature effect on enzymes & deactivation kinetics.

UNIT III ENZYME IMMOBILIZATION AND BIOSENSORS

Physical and chemical techniques for enzyme immobilization – adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc., – examples, advantages and disadvantages, design of enzyme electrodes and their application as biosensors in industry, healthcare and environment.

UNIT IV PURIFICATION AND CHARACTERIZATION OF ENZYMES FROM NATURAL SOURCES

Production and purification of crude enzyme extracts from plant, animal and microbial sources; methods of characterization of enzymes; development of enzymatic assays

UNIT V BIOTRANSFORMATION APPLICATIONS OF ENZYMES

Hydrolytic- Ester bond, Amide, Epoxides, Nitriles, Reduction reactions –aldehydes, Ketones, C=C, Oxidation reactions – Alkanes, Aromatic, Baeyer-Villiger, Enzymes in organic synthesis – esters, amide, peptide , Modified and Artificial Enzymes , Catalytic antibodies

TOTAL: 45 PERIODS

COURSE OUTCOMES:

On completion of the course, the students would have the knowledge on
 On enzyme and enzyme reactions will be the key step in to proceed towards various concepts in biotechnology.
 Theoretical and practical aspects of kinetics will provide the importance and utility of enzyme kinetics towards research.
 Process of immobilization has been increased steadily in food, pharmaceutical and chemical industries and thus this study will provide simple and easy method of implementation.
 Ideas on Processing, Production and Purification of enzymes at an industrial scale will be helpful to work technologically.

TEXT BOOKS:

1. Trevor Palmer , 5th edition Enzymes Horwood Publishing Ltd, 2001
2. Faber K , Biotransformations in Organic Chemistry, 2nd Edition , Springer

REFERENCES:

1. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, 2nd Edition, CRC Press, 1997
2. James M. Lee, Biochemical Engineering, PHI, USA.
3. James. E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, 2nd Edition, McGraw Hill Education; 2017.
4. Wiseman, Enzyme Biotechnology, Ellis Horwood Pub. Volume 4, 1980