BT3511 Molecular Biolog and Genetic Engineering Laboratory Syllabus:

BT3511 Molecular Biolog and Genetic Engineering Laboratory Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES:

 Provide hands-on experience in performing basic molecular biology and Genetic Engineering techniques.
 Introduce the theory behind in each technique and to describe common applications of each methodology in biological research. This will facilitate the students to take up specialized project in Molecular biology and will be a pre-requisite for research work.

LIST OF EXPERIMENTS

1. Isolation of total DNA
2. Isolation of Plasmid DNA
3. Agarose gel electrophoresis
4. Restriction enzyme digestion of DNA
5. DNA ligation
6. Competent cell preparation & Transformation
7. Blue-White screening
8. Induction and Analysis of Gene expression
9. PCR
10. SDS-PAGE
11. Western blot

TOTAL: 60 PERIODS

COURSE OUTCOMES

Upon completion of the course, students will be able to
 Understand basic techniques of DNA isolation and manipulation.
 Gain experience in selecting genetically transformed organisms for downstream analysis.
 Aware basic techniques involved in analysis of gene expression at nucleic acids and proteins level
 Establish the ability to carry out laboratory experiments and interpret the results.
 Apply practical knowledge to solve biotechnological problems.
 Aware of the hazardous chemicals and safety precautions in case of emergency.

TEXT BOOKS:

1. Sue Carson, Heather Miller, Melissa Srougi, D. Scott Witherow, “Molecular Biology Techniques: A Classroom Laboratory Manual”, Fourth Edition, Academic Press, Elsevier, 2019.
2. Frank H. Stephenson, “Calculations for Molecular Biology and Biotechnology” Third Edition, Academic Press, Elsevier, 2016.
3. Michael R. Green and Joseph Sambrook, “Molecular Cloning: A Laboratory Manual”, Fourth Edition, Cold Spring Laboratory Press, 2012.

REFERENCES:

1. Keith Wilson and John Walker, “Principles and Techniques of Biochemistry and Molecular Biology” 8th Edition, Cambridge University Press, 2018.
2. Alan S. Gerstein, “Molecular Biology Problem Solver-A Laboratory Guide” Wiley-Liss, Inc., 2017.