BT3661 Bioprocess Laboratory Syllabus:

BT3661 Bioprocess Laboratory Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES

 To provide a practical exposure to the bioinformatics databases, tools and software.
 To select and use functions in Perl for processing sequence data.

EXPERIMENTS

1. Enzyme kinetics – Determination of Michaelis – Menten parameters
2. Enzyme activity – Effect of Temperature and Deactivation Kinetics
3. Enzyme activity – Effect of pH
4. Enzyme inhibition kinetics
5. Enzyme immobilization – Gel entrapment
6. Enzyme immobilization –Cross-linking
7. Enzymatic conversion in Packed bed Column
8. Growth of Bacteria – Estimation of Biomass, Calculation of Specific Growth Rate, Yield Coefficient
9. Optimization by Plackett Burman Design
10. Optimization by Response Surface Methodology
11. Estimation of KLa – Dynamic by different methods
12. Estimation of Overall Heat Transfer Coefficient and mixing time in reactor

TOTAL: 45 PERIODS

OUTCOMES:

At the end of this course, students will be able to:
 Explain about Enzyme kinetics and characterization and how to use them for practical applications.
 Evaluate the growth kinetics of microorganisms and become adept with medium optimization techniques.
 Determine an experimental objective, understand the theory behind the experiment, and operate the relevant equipment safely.
 Demonstrate good lab citizenry and the ability to work in team

REFERENCES:

1. Peter F. Stanbury, Stephen J. Hall & A. Whitaker, Principles of Fermentation Technology, Butterworth-Heinemann; 3rd Edition ,2016.
2. Pauline M. Doran, Bioprocess Engineering Principles, Elsevier, 2nd Edition,2012
3. Michael L. Shuler, Fikret Kargi, Matthew De Lisa , Bioprocess Engineering , Prentice Hall, 3rd Edition, 2017
4. Bailey, James E. and David F. Ollis, “ Biochemical Engineering Fundamentals”, 2ndEdition. McGraw Hill , 1986.