BT3004 Bioreactor Design and Scale up Process Syllabus:

BT3004 Bioreactor Design and Scale up Process Syllabus – Anna University Regulation 2021

UNIT I BASIC BIOREACTOR CONCEPTS

Bioreactor Operation – Batch operation, semi-continuous and fed-batch operation, Continuous Operation – Chemostat, turbidostat – Microbiological reactors, enzyme reactors – Tank-type,
Column-type biological reactors – Case studies – Continuous Fermentation with Biomass Recycle, Tanks-in-series, Tubular plug flow bioreactors.

UNIT II AERATION AND AGITATION IN BIOPROCESS SYSTEMS

Mass transfer in agitated tanks; Power requirement for mixing; Agitation rate studies – Mixing time and residence time distribution; Bioreactor Geometry – Reactor, impeller, sparger and baffle design; shear damage, bubble damage, methods of minimizing cell damage. Case Studies for Aeration and Agitation

UNIT III SELECTION AND DESIGN OF BIOPROCESS EQUIPMENT

Materials of construction for bioprocess plants – Design considerations for maintaining sterility of process streams processing equipments, selection, specification – Design of heat and mass
transfer equipment used in bioprocess industries.

UNIT IV BIOREACTOR SCALE-UP AND SCALE-DOWN

Scale-up Techniques: – Scale up by geometric similitude. constant power consumption per volume, constant mixing time, constant impeller tip speed, constant volumetric mass transfer coefficient; Scale–down Related Aspects; Case Studies in Bioreactor Scaleup and Scale-down Aspects

UNIT V CASE STUDIES

Requirements, design and operation of bioreactor for microbial, plant cell and animal cell.

TOTAL 45 PERIODS

TEXT BOOKS

1. Michael L. Shuler, Fikret Kargi, Matthew De Lisa,.Bioprocess Engineering, 3rd Edition,Prentice Hal, 2017
2. Pauline Doran, Bioprocess Engineering Calculation, 2nd Edition, Blackwell Scientific Publications,2012
3. James M. Lee, Biochemical Engineering, Prentice Hall, 1992

REFERENCES

1. James E. Bailey and David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill 1986.
2. S.Liu, Bioprocess Engineering: Kinetics, Biosystems, Sustainability, and Reactor Design, Elsevier, 2016
3. Octave Levenspiel, Chemical Reaction Engineering, Wiley 2016.