BT3008 Stem Cell Technology Syllabus:
BT3008 Stem Cell Technology Syllabus – Anna University Regulation 2021
UNIT I INTRODUCTION TO STEM CELLS
Stem cell Classification, Sources and Properties –Types of stem cells: methods of isolation, study of stem cells and their viability IPSC, embryonic stem cells, cancer stem cells. – Preservations of Stem cell. Embryonic stem cell: Isolation, Culturing, Differentiation, Properties – Adult stem cell: Isolation, Culturing, Differentiation, Trans-differentiation, Plasticity, and Properties
UNIT II HUMAN EMBRYONIC AND ADULT STEM CELL
Stem cells and their developmental potential. In vitro fertilization-culturing of embryos – blastocystinner cell mass-isolation and growing ES cells in lab; Identification and characterization of human ES cells. Somatic stem cells-test for identification of adult stem cells- adult stem cell differentiationtrans differentiation-plasticity-different types of adult stem cells-liver stem cells-skeletal muscle stem cells-bone marrow derived stem cells.
UNIT III DIFFERENTIATION OF STEM CELLS INTO CELL TYPES
Factors influencing cell specialization – internal factors – asymmetric segregation, cell signaling mechanisms – diffusion, direct contact and gap junctions; environmental factors – temperature, drugs and injuries; mechanism of stem cell differentiation – errors in cell differentiation – anaplasia, dysplasia and metaplasia.
UNIT IV STEM CELLS IN TISSUE ENGINEERING
Haematopoietic Stem Cells-Growth factors and the regulation of haematopoietic stem cells, clinical applications of haematopoietic stem cells; HLA matching, patient selection, peripheral blood and bone marrow transplantation; Mesenchymal stem cells and their role in bone tissue engineeringbone repair; Stem cell based gene therapy and benefits to human. Techniques in stem cell technology – fluorescence activated cell sorting (FACS), time lapse video, green fluorescent protein tagging
UNIT V APPLICATION AND ETHICAL ISSUES
Therapeutic applications-Parkinsons disease, Cancer stem cell – Neural stem cell for central nervous system repair – Spinal cord injury – use of ESC to treat heart disease – Burns and skin
ulcers – Orthopaedic applications of stem cell – Insulin-producing Cells Derived from Stem cells: A Potential Treatment for Diabetes; Stem cell policy and ethics, stem cell research: Hype, hope and controversy.
TOTAL: 45 PERIODS
Course outcomes:
To differentiate different types of stem cells and to characterize them
To gain knowledge on animal and plant stem cells
To develop techniques to program stem cells into specific cell types
To determine the factors affecting stem cell differentiation
To understand the role of stem cells in tissue engineering and regenerative medicine
To become familiarized with stem cell technology and its applications for the betterment of society
TEXT BOOKS:
1. Stem cells by C.S Potten., Elsevier, 2006.
2. Essentials of Stem Cell Biology by Robert Lanza., fourth edition. Elsevier 2014.
REFERENCES
1. Stem cell biology and Gene Therapy by Peter Quesenberry., First Edition, Wiley-Liss, 1998.
2. Embryonic Stem cells – Protocols by KursadTurksen., Second Edition Humana Press, 2002.
3. Stem Cells: From Bench to Bedside by AriffBongso, EngHinLee., World Scientific Publishing Company,2005.
4. Stem cells in clinic and Research by Ali Gholamrezanezhad., Intech, 2013