BT3351 Cell Biology Syllabus:
BT3351 Cell Biology Syllabus – Anna University Regulation 2021
COURSE OBJECTIVES:
To provide knowledge on the fundamentals of cell biology
To help students understand the signalling mechanisms
UNIT I CELL STRUCTURE AND FUNCTION OF THE ORGANELLES
Prokaryotic, Eukaryotic cells, Sub-cellular organelles and functions. Principles of membrane organization membrane proteins, cytoskeletal proteins. Extra cellular matrix, cell-cell junctions.
UNIT II CELL DIVISION, CANCER, APOPTOSIS AND IMMORTALIZATION OF CELLS
Cell cycle – Mitosis, Meiosis, Molecules controlling cell cycle, cancer, role of Ras and Raf in oncogenesis and apoptosis. Stem cells, Cell culture and immortalization of cells and its applications.
UNIT III TRANSPORT ACROSS CELL MEMBRANE
Passive and Active Transport, Permeases, Ion channels, ATP pumps. Na+ / K+ /Ca+2Tpumps, uniport, symport antiporter system. Ligand gated / voltage gated channels, Agonists and Antagonists.
UNIT IV SIGNAL TRANSDUCTION
Receptors – extracellular signaling, Cell surface / cytosolic receptors and examples, Differentclasses of receptors antocrine / paracrine / endocrine models, Secondary messengers molecules.
UNIT V TECHNIQUES USED TO STUDY CELLS
Cell fractionation and flow cytometry, Morphology and identification of cells using microscopic studies like SEM, TEM and Confocal Microscopy. Localization of proteins in cells – Immunostaining.
TOTAL: 45 PERIODS
COURSE OUTCOMES:
Upon completion of this course, the students
To have deeper understanding of cell at structural and functional level
To understand the structures and purposes of basic components of prokaryotic and eukaryotic cells
To become familiar on the use of cellular components to generate and utilize energy in cells
To possess broad knowledge on the molecular interaction between cells.
To demonstrate a clear understanding between secondary messengers and signal transduction mechanism
To develop skill on working principles of microscopy and identification of cell types.
TEXT BOOKS:
1. Lodish, Harvey etal., “Molecular Cell Biology”, 7th Edition, W.H.Freeman, 2013.
2. Cooper, G.M. and R.E. Hansman “The Cell: A Molecular Approach”, 8th Edition, Oxford University Press, 2018.
3. Alberts, Bruce etal., “Molecular Biology of the Cell”, 6th Edition, W.W. Norton,2014
4. Sadava, D.E. “Cell Biology: Organelle Structure and Function”, Panima Publishing, 2004.
5. Rastogi, S.C. “Cell Biology” 2nd Edition, New Age International, 2017
REFERENCES:
1. Becker, W.M. etal., “The World of the Cell”, 9th Edition, Pearson Education, 2003.
2. Campbell, N.A., J.B. Recee and E.J. Simon “Essential Biology”, VIIrd Edition, Pearson International, 2007.
3. Alberts, Bruce etal., “Essential Cell Biology”, 4th Edition, W.W. Norton, 2013