BT3362 Cell and Microbiology Laboratory Syllabus:

BT3362 Cell and Microbiology Laboratory Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES:

 To demonstrate various techniques to learn the morphology, identification and propagation of cells and microbes.
 To learn the staining techniques and culturing of microorganism.

EXPERIMENTS

1. Introduction, Laboratory Safety, Use of Equipment; Sterilization Techniques
2. Microscopy – Working and care of Microscope, phase contrast and fluorescent microscopy
3. Culture Media-Types and Use; Preparation of Nutrient broth and agar
4. Culture Techniques, Isolation and Preservation of Cultures- Broth: flask, test tubes; Solid:Pour plates, streak plates, slants, stabs
5. Identification of given plant, animal, bacterial cells and yeast/mould
6. Staining Techniques Simple, Differential- Gram’s Staining, spore /capsule staining, Giemsa, and Leishman Staining
7. Quantification of Microbes: Sampling and Serial Dilution; Bacterial count in Soil – TVC
8. Effect of Disinfectants- Phenol Coefficient, Antibiotic Sensitivity Assay
9. Osmosis and Tonicity and Tryphan Blue Assay
10. Growth Curve in Bacteria and Yeast
11. Staining for different stages of mitosis in AlliumCepa (Onion)
12. Effect of pH, Temperature, UV radiation on Growth Bacteria

TOTAL: 45 PERIODS

COURSE OUTCOMES:

Students will be able to
 Understand the advanced technical information pertaining to laboratory bio-safety and preventive measures from pathogenic microorganism.
 Know the various aseptic techniques and sterilization methods.
 Develop the minimum skills to work on several important techniques for the study of microorganisms in the laboratory.
 Learn the various techniques of culturing of microorganisms and media preparation.
 Study the growth of microorganisms by varying the growth conditions.
 Identify the various stages of mitosis

REFERENCES:

1. Cappuccino, J.G. and N. Sherman “Microbiology: A Laboratory Manual”, 4th Edition, Addison-Wesley, 1999.
2. Collee, J.G. etal., “Mackie & McCartney Practical Medical Microbiology” 4th Edition, ChurchillLivingstone, 1996Rickwood, D. and J.R. Harris “Cell Biology: Essential Techniques”, Johnwiley, 1996.
3. Davis, J.M. “Basic Cell Culture: A Practical Approach”, IRL, 1994.