BT3761 Downstream Laboratory Syllabus:

BT3761 Downstream Laboratory Syllabus – Anna University Regulation 2021

OBJECTIVES:

 To provide hands on training in Down stream processing through simple experimentations inthe laboratory. This will be a pre-requisite for project work.
 The objectives of this course is to practice the students
 To understand the nature of the end product, its concentration, stability and degree of purification required
 To design processes for the recovery and subsequent purification of target biological products.

EXPERIMENTS

1. Solid liquid separation – centrifugation
2. Solid liquid separation – microfiltration
3. Cell disruption techniques – ultrasonication or French pressure cell or Dynomill
4. Precipitation – ammonium sulphite precipitation
5. Ultra filtration separation
6. Aqueous two phase extraction of biologicals
7. High resolution purification – affinity chromatography
8. High resolution purification – ion exchange chromatography
9. Product polishing – spray drying or freeze drying
10. Size exclusion chromatography

TOTAL: 45 PERIODS

OUTCOMES:

Upon success completion of this course, the students would have
1. Acquired knowledge for the separation of whole cells and other insoluble ingredients from the culture broth.
2. Learned cell disruption techniques to release intracellular products
3. Learned various techniques like evaporation, extraction, precipitation, membrane separation for concentrating biological products
4. Learned the basic principles and techniques of chromatography to purify the biological products and formulate the products for different end uses.

REFERENCES:

1. P.A. Belter, E.L. Cussler And Wei-Houhu – Bioseparations – Downstream Processing For Biotechnology, Wiley Interscience Pun. (1988).
2. J.C. Janson And L. Ryden, (Ed.) – Protein Purification – Principles, High ResolutionMethods And Applications, VCH Pub. 1989.
3. Michael R.Ladisch Bioseparations Engineering: Principles, practice and Economics, WileyInterscience,1st Edition,2001.
4. Product Recovery in Bioprocess Technology”. (BIOTOL – Biotechnology by Open Learning Series). Butterworth – Heinmann / Elsevier, 2004.
5. Raja Ghosh “Principles of Bioseparations Engineering”. World Scientific, 2006