BT3361 Biochemistry Laboratory Syllabus:

BT3361 Biochemistry Laboratory Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES

The student should made to
 Learn fundamental approaches for experimentally investigating biochemical problems.
 Able to extract living cell samples from plants and animals for genetic research

EXPERIMENTS

1. General guidelines for working in biochemistry lab (theory)
2. Units of volume, weight, density and concentration measurements and their range in biological measurements. Demonstration of proper use of volume and weight measurement devices.
3. Accuracy, precision, sensitivity and specificity (theory)
4. Preparation of buffer –titration of a weak acid and a weak base.
5. Qualitative tests for carbohydrates – distinguishing reducing from non-reducing sugars and keto from aldo sugars.
6. Quantitative method for amino acid estimation using ninhydrin – distinguishing amino from imino acid.
7. Protein estimation by Biuret and Lowry’s methods.
8. Protein estimation by Bradford and spectroscopic methods.
9. Extraction of lipids and analysis by TLC.
10. Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).
11. Enzymatic assay: phosphatase from potato.
12. Enzymatic assay: estimation of glucose by GOD-POD method after hydrolysis of starch with acid and specificity of the enzymatic method.

TOTAL: 45 PERIODS

COURSE OUTCOMES

Upon completion of this course the student will be able to
 Analyze current biochemical and molecular techniques to plan and carry out experiments.
 Perform good biochemical laboratory practices.
 Adapt methods for biochemical analysis.
 Carry out experiments in biomolecular separations.
 Learn and understand the principles behind the qualitative and quantitative estimation of biomolecules.
 Understand the applicability of biochemical methods to realistic solution.

TEXT BOOKS

1. Practical Biochemistry by R.C. Gupta and S. Bhargavan.
2. Introduction of Practical Biochemistry by David T. Phummer. (II Edition)

REFERENCES

1. Harpers Biochemistry Ed. R.K. Murray , D.K. Granner, P.A. Mayes and V.W.Rodwell, Appleton and Lange ,Stanford ,Conneticut.
2. Textbook of Biochemistry with clinical correlations. Ed. Thomas M. Devlin. Wiley Liss Publishers