BT3451 Analytical Techniques in Biotechnology Syllabus:
BT3451 Analytical Techniques in Biotechnology Syllabus – Anna University Regulation 2021
COURSE OBJECTIVES:
To enable the students
To have a fundamental knowledge about the Light spectrum, Absoprtion, Fluorescence, NMR, Mass spectroscopy
To acquire knowledge on the different chromotographic methods for separation of biological products.
UNIT I INTRODUCTION TO SPECTROMETRY
Properties of electromagnetic radiation- wave properties – components of optical instruments – Sources of radiation – wavelength selectors – sample containers – radiation transducers – Signal process and read outs – signal to noise ratio – sources of noise – Enhancement of signal to noise – types of optical instruments – Principle of Fourier Transform optical Measurements.
UNIT II MOLECULAR SPECTROSCOPY
Molecular absorption spectrometry – Measurement of Transmittance and Absorbance – Beer’s law – Instrumentation – Applications -Theory of fluorescence and Phosphorescence – Instrumentation – Applications – Theory of Infrared absorption spectrometry – IR instrumentation – Applications – Theory of Raman spectroscopy – Instrumentation – applications
UNIT III MAGNETIC RESONANCE SPECTROSCOPY AND MASS SPECTROMETRY
Theory of NMR – environmental effects on NMR spectra – chemical shift- NMR-spectrometers – applications of 1H and 13C NMR- Molecular mass spectra – ion sources – Mass spectrometer. Applications of molecular mass – Electron paramagnetic resonance- g values –instrumentation.
UNIT IV SEPARATION METHODS
General description of chromatography – Band broadening and optimization of column performance- Liquid chromatography – Partition chromatography – Adsorption chromatography –
Ion exchange chromatography -size exclusion chromatography- Affinity chromatography- principles of GC and applications – HPLC- Capillary electrophoresis – Applications.
UNIT V ELECTRO ANALYSIS AND SURFACE MICROSCOPY
Electrochemical cells- Electrode potential cell potentials – potentiometry- reference electrode – ion selective and molecular selective electrodes – Instrument for potentiometric studies – Voltametry – Cyclic and pulse voltametry- Applications of voltametry . Study of surfaces – Scanning probe microscopes – AFM and STM.
TOTAL: 45 PERIODS
COURSE OUTCOME:
On completion of the course, students will have a better understanding of spectroscopy and the separation techniques used for biological products.
TEXT BOOKS:
1. Skoog, D.A. F. James Holler, and Stanky, R.Crouch “Instrumental Methods of Analysis”.CengageLearning , 2007.
2. Willard, Hobart, etal., “Instrumental Methods of Analysis”. VIIth Edition, CBS, 1986.
3. Fifield F.W., . Principles and Practice of Analytical Chemistry. Blackwell, Scientific Publishers,2016
REFERENCES:
1. Sharma, B.K. “Instrumental Methods of Chemical Analysis: Analytical Chemistry”, Krishna Prakashan Media (P) Ltd, 2014
2. Haven, Mary C., etal., “Laboratory Instrumentation “. 4th Edition, Wiley India Pvt Ltd, 2010
3. Philopose P.M.Analytical Biotechnology. Domihant Publishers & distributors, New Delhi, 2016.