BT3005 Bioreactor Considerations for Recombinant Products Syllabus:
BT3005 Bioreactor Considerations for Recombinant Products Syllabus – Anna University Regulation 2021
OBJECTIVE:
To expose students to application of recombinant DNA technology in biotechnological research.
To train students in strategizing research methodologies employing genetic engineering techniques.
UNIT I GENETICALLY ENGINEERED ORGANISMS
Different host vector systems, Guidelines for choosing Host Vector systems, Process constraints – Genetic instability, considerations in plasmid design, Regulatory constraints, principles and implementation of containment, good industrial large-scale practice (GILSP).
UNIT II CONSIDERATIONS FOR ANIMAL CELL CULTURES
Structure and biochemistry of animal cells – Methods Used for the cultivation of animal cells – Bioreactor considerations for animal cell culture – Products of animal cell cultures, economics of animal cell tissue cultures.
UNIT III CONSIDERATIONS FOR PLANT CELL CULTURES
Overview of plant cell cultures – Plant cells in culture compared to microbes – Bioreactor considerations for plant cell culture – Bioreactors for suspension cultures – Reactors using cell immobilization – Bioreactors for organized tissues, economics of plant cell tissue cultures.
UNIT IV DOWNSTREAM PROCESSING CONSIDERATIONS
Release of protein from Biological Host, genetic approaches to facilitate protein purification, SolidLiquid separation, extraction of Recombinant protein, Avoidance of proteolysis from extracts, membranes for protein isolation and purification, Chromatographic techniques, Removal of detergent from protein fractions, precipitation of proteins, protein crystallization for large scale bio separation.
UNITV SAFETY CONSIDERATIONS ASSOCIATED WITH AGRICULTURAL AND ENVIRONMENTAL APLLICATIONS
Risk assessment methods, safety considerations, Application of rDNA organism in the environment, Survival, multiplication and/or dissemination in the environment, Interactions with
species or biological systems, effects on the environment, evaluating environmental risks of rDNA organisms released from industrial applications.
TOTAL 45 PERIODS
COURSE OUTCOMES:
Upon completion of this course the student will be able to
To acquire skills on techniques of isolation of gene of interest and construction of recombinant DNA.
To apply techniques for production of pharmaceuticals, growth hormones, vaccines, gene therapy in expression system.
To apply rDNA technology in evolving plants for resistance to pest and disease, tolerance to herbicides and abiotic factors.
To identify problems associated with production of recombinant proteins and protein purification and devising strategies to overcome problem.
To acquire knowledge on environmental applications of genetic engineering through bioremediation.
To identify the methods for selection of recombinants and to express recombinant protein in E. coli and eukaryotes.
TEXT BOOKS:
1. Michael L. Shuler, Fikret Kargi, Matthew De Lisa,.Bioprocess Engineering, 3rd Edition,Prentice Hal, 2017
2. Bailey J.A and Ollis D.F., “Biochemical Engineering Fundamentals”, McGraw Hill (New York), 2nd Edition, 2010.
3. Cutler, P. ed., 2004. Protein purification protocols (Vol. 244). Springer Science & Business Media.
4. Perry R H ,“Perry’s Chemical Engineers’ Handbook”, McGraw-Hill, 8th Edition, 2008.
REFERENCES:
1. Pörtner, R. and Barradas, O.B.J.P., 2007. Animal cell biotechnology. Methods and Protocols, 2nd. Edition. Humana.
2. Slater, A., Scott, N. and Fowler, M., 2008. Plant biotechnology: the genetic manipulation of plants. OUP Oxford.
3. Pörtner, R. and Barradas, O.B.J.P., 2007. Animal cell biotechnology. Methods and Protocols, 2nd. Edition. Humana.
4. Slater, A., Scott, N. and Fowler, M., 2008. Plant biotechnology: the genetic manipulation of plants. OUP Oxford.