BT3651 Bioprocess Engineering Syllabus:

BT3651 Bioprocess Engineering Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES:

The student should be made to,
 To provide the students with the basics of bioreactor engineering.
 To develop bioengineering skills for the production of biochemical product using integrated biochemical processes.

UNIT I CONFIGURATION OF BIOREACTORS

Ideal reactors and its characteristics Fed batch cultivation, Cell recycle cultivation, Cell recycle cultivation in waste water treatment, two stage cultivation Packed bed reactor, airlift reactor,
introduction to fluidized bed reactor bubble column reactors

UNIT II BIOREACTOR SCALE – UP

Regime analysis of bioreactor processes, oxygen mass transfer in bioreactors – microbial oxygen demands; methods for the determination of mass transfer coefficients; mass transfer correlations. Scale up criteria for bioreactors based on oxygen transfer, power consumption and impeller tip speed.

UNIT III BIOREACTOR CONSIDERATION IN ENZYME SYSTEMS

Analysis of film and pore diffusion effects on kinetics of immobilized enzyme reactions; formulation of dimensionless groups and calculation of effectiveness factors. Design of immobilized enzyme reactors – packed bed, fluidized bed and membrane reactors

UNIT IV MODELLING AND SIMULATION OF BIOPROCESSES

Study of structured models for analysis of various bioprocess – compartmental models, models of cellular energetics and metabolism, single cell models, plasmid replication and plasmid stability model. Dynamic simulation of batch, fed batch, steady and transient culture metabolism.

UNIT V RECOMBINANT CELL CULTIVATION

Different host vector system for recombinant cell cultivation strategies and advantages. E.coli, yeast Pichia pastoris/ Saccharomyces cereviseae, Animal cell cultivation, plant cell cultivation,
Insect cell cultivation. High cell density cultivation, process strategies, reactor considerations in the above system

TOTAL: 45 PERIODS

OUTCOMES:

Upon completion of this course the student will be able to
 Describe various bioreactor configurations and operation modes.
 Apply the knowledge of bioreactor scale up on the basis of rule of thumbs.
 Define kinetic parameters and apply the bioreactor considerations for immobilized enzymes systems.
 Utilize modelling approaches and simulation concepts for bioprocess estimations.
 Apply bioreactor considerations for the development of recombinant products.
 Explore the engineering concepts of bioreactors.

TEXT BOOKS:

1. Michael L. Shuler, Fikret Kargi, Matthew De Lisa,.Bioprocess Engineering, 3rdEdition,Prentice Hal, 2017
2. Pauline Doran, Bioprocess Engineering Calculation, 2nd Edition, Blackwell Scientific Publications,2012.
3. Sarfaraz K. Niazi, Justin L. Brown , Fundamentals of Modern Bioprocessing, Taylor & Francis,2017

REFERENCES

1. Anton Moser, “Bioprocess Technology:Kinetics and Reactors”, , Springer Verlag.2012
2. James E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill,1986
3. James M. Lee, Biochemical Engineering, PHI, USA.1992
4. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, CRC Press,1997