EN3411 Environmental Chemistry and Microbiology Laboratory Syllabus:

EN3411 Environmental Chemistry and Microbiology Laboratory Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES:

• To train in the analysis of physico-chemical parameters with hands on experience
• To train the students in the analysis of various microbiological techniques, microbiological analysis, enzyme assay, pollutant analysis and operation of bioreactors.

A: Environmental Chemistry
1. Estimation of hardness in Water sample by volumetric titration
2. Estimation of Chloride in Water sample by volumetric titration
3. Determination of sulphate
4. Determination of phosphate
5. Determination of Total Solids, Total suspended solids, Total dissolved solids
6. Determination of COD in the wastewater sample
7. Determination of BOD in the wastewater sample

B: Environmental Microbiology
1. Preparation of culture media
2. Isolation and Culturing of Microorganisms
3. Gram Staining of bacteria
4. Bacteriological analysis of wastewater (Coliforms & Streptococcus) – MPN Technique
5. Bacteriological analysis of wastewater (Coliforms & Streptococcus MF technique

TOTAL: 60 PERIODS
COURSE OUTCOMES

CO1 Analyze the water quality parameters such as hardness chloride and sulphate
CO2 Characterize the wastewater parameters like phosphate, solids COD and BOD
CO3 Prepare culture media necessary for microbial growth
CO4 Isolate and culture the bacteria – identify the bacteria -able to handle microscope
CO5 Analyze the coliform count in the wastewater.

REFERENCES

1. APHA, “Standard Methods for the Examination of Water and Wastewater”, 23rd Ed. Washington, 2017
2. “Laboratory Manual for the Examination of water, wastewater soil, Rump, H.H. and Krist, H. – Second Edition, VCH, Germany, 3rd Edition, 1999.
3. Charles P. Gerba, “Environmental Microbiology: A laboratory manual”, Elsevier Publications, 3rd, 2014