CBM349 Genetic Engineering Syllabus:
CBM349 Genetic Engineering Syllabus – Anna University Regulation 2021
COURSE OBJECTIVES:
To discuss the gene cloning methods and the tools and techniques involved in gene cloning and genome analysis and genomics.
To explain the heterologous expression of cloned genes in different hosts.
UNIT I BASICS OF RECOMBINANT DNA TECHNOLOGY
Manipulation of DNA – Restriction and Modification enzymes – Design of linkers and adaptors – Characteristics of cloning and expression vectors – Introduction of recombinant DNA in to host cells and selection methods.
UNIT II DNA LIBRARIES
Construction of genomic and cDNA libraries, Artificial chromosomes – Bacteria, Yeast – Chromosomal walking.
UNIT III SEQUENCING AND AMPLIFICATION OF DNA
Maxam Gilbert’s and Sanger’s methods of DNA sequencing – PCR: Inverse PCR, Nested PCR, Allele specific PCR, Hot start PCR, Colony PCR, single cell PCR, Real-time PCR/qPCR – SYBR green assay, Taqman assay, Molecular beacons. Site directed mutagenesis.
UNIT IV ORGANIZATION AND STRUCTURE OF GENOMES
Organization and structure of genomes – Genome sequencing methods: Conventional and shotgun genome sequencing methods, Next generation sequencing technologies – Ordering the genome sequence – Genetic maps and Physical maps, STS content based mapping, Hybridization mapping, Optical mapping.
UNIT V CURRENT STATUS OF GENOME SEQUENCING PROJECTS
Introduction to Functional genomics – Microarrays – Serial Analysis of Gene expression (SAGE), Subtractive hybridization, Comparative Genomics, Proteogenomics, Web resources for Genomics, Applications of genome analysis and genomics.
COURSE OUTCOMES:
At the end of the course, the student should be able to:
CO1: Would be aware of how to clone commercially important genes.
CO2: The students would be aware of how to produce the commercially important recombinant proteins.
CO3: Will be familiarized with gene and genome sequencing techniques
CO4: Will be aware of microarrays, Analysis of Gene expression and proteomics.
CO5: Acquire ability to function on multi-disciplinary teams
TOTAL:45 PERIODS
TEXT BOOK
1. Old RW, Primrose SB, “Principles of Gene Manipulation, An Introduction to Genetic Engineering”, Blackwell Science Publications, 1993.
2. Principles of Genome Analysis and Genomics by S.B.Primrose and R.M.Twyman, 3rd Ed. (Blackwell Publishing).
REFERENCES
1. Isil Aksan Kurnaz, “Techniques in Genetic Engineering”, CRC Press, 2015.
2. Oksana Ableitner, “Introduction to Molecular Biology: Working with DNA and RNA (essentials)”, Springer International, 2022.
3. Arun K. Shukla, “Proteomics in Biology”, Academic Press, 2017.