BT3392 Biochemistry Syllabus:
BT3392 Biochemistry Syllabus – Anna University Regulation 2021
OBJECTIVE
To enable students learn the fundamentals of Biochemical Processes and Biomolecules
UNIT I INTRODUCTION TO BIOMOLECULES – CARBOHYDRATES
Basic principles of organic chemistry, role of carbon, types of functional groups, chemical, nature of water, pH and biological buffers, bio molecules structure and properties of Carbohydrates (mono, di, oligo & polysaccharides) Proteoglycans, glucosaminoglycans. mutarotation, glycosidic bond, reactions of monosaccharides, reducing sugars. Starch, glycogen, cellulose and chitin. Proteoglycans, glycosaminoglycans. hyaluronic acid, chondroitin sulfate
UNIT II STRUCTURE AND PROPERTIES OF OTHER BIOMOLECULES
Structure and properties of Important Biomolecules. Lipids: fatty acids, glycerol, saponification, iodination, hydrogenation, phospholipids, glycolipids, sphingolipids, cholesterol, steroids, prostaglandins.
Protein: Amino Acids, Peptides, Proteins, measurement, structures, hierarchy of organization primary, secondary, tertiary and quaternary structures, glycoproteins, lipoproteins. Determine of primary structure.
Nucleic acids: purines, pyrimidines, nucleoside, nucleotide, RNA, DNA-Watson-Crick structure of DNA, reactions, properties, measurement, nucleoprotein complexes
UNIT III METABOLISM CONCEPTS AND CARBOHYDRATE METABOLISM
Functions of Proteins, Enzymes, introduction to biocatalysts, metabolic pathways, primary and secondary metabolites. Interconnection of pathways and metabolic regulation. Glycolysis, TCA cycle, gluconeogenesis, pentose phosphate shunt & glyoxalate shunt.
UNIT IV INTERMEDIARY METABOLISM AND REGULATION
Fatty acid synthesis and oxidation, reactions of amino acids, deamination, transamination and decarboxylation, urea cycle, Bioenergetics – High energy compounds, electronegative potential of compounds, respiratory chain, ATP cycle, calculation of ATP yield during oxidation of glucose and fatty acids.
UNIT V PROTEIN TRANSPORT AND DEGRADATION
Protein targeting, signal sequence, secretion; Folding, Chaperone and targeting of organelle proteins, Protein degradation, receptor-mediated endocytosis, turnover.
TOTAL: 45 PERIODS
OUTCOMES
On completion of the course, the students will be able to
CO1 – understand the basic concepts on carbohydrates.
CO2 – learn the concepts of proteins
CO3 – gather knowledge on importance of nucleic acids
CO4 – understand the knowledge on lipids
CO5 – gather knowledge on intermediary metabolism and their pathways
TEXT BOOKS
1. Lehninger Principles of Biochemistry 6th Edition by David L. Nelson, Michael M. Cox W.H.Freeman and Company 2017
2. Satyanarayana, U. and U. Chakerapani, “Biochemistry” 3rd Rev. Edition, Books & Allied (P) Ltd., 2006.
3. Rastogi, S.C. “Biochemistry” 2nd Edition, Tata McGraw-Hill, 2003.
4. Conn, E.E., etal., “Outlines of Biochemistry” 5th Edition, John Wiley & Sons, 1987.
5. Outlines of Biochemistry, 5th Edition: By E E Conn, P K Stumpf, G Bruening and R Y Doi. pp 693. John Wiley and Sons, New York. 1987.
REFERENCES
1. Berg, Jeremy M. et al. “Biochemsitry”, 6th Edition, W.H. Freeman & Co., 2006.
2. Murray, R.K., etal “Harper’s Illustrated Biochemistry”, 31st Edition, McGraw-Hill, 2018.
3. Voet, D. and Voet, J.G., “Biochemistry”, 4th Edition, John Wiley & Sons Inc.,2010.