BT3551 Bioprocess Principles Syllabus:

BT3551 Bioprocess Principles Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES:

 To impart knowledge on design and operation of fermentation processes with all its prerequisites.
 To endow the students with the basics of microbial kinetics, metabolic stoichiometry and energetics.

UNIT I OVERVIEW OF FERMENTATION PROCESSES

Overview of fermentation industry, general requirements of fermentation processes, basic configuration of fermentor (CSTR) and ancillaries, main parameters to be monitored and controlled in fermentation processes.

UNIT II RAW MATERIALS AND MEDIA DESIGN FOR FERMENTATION PROCESS

Criteria for good medium, medium requirements for fermentation processes, carbon, nitrogen, minerals, vitamins and other complex nutrients, oxygen requirements, medium formulation of optimal growth and product formation, examples of simple and complex media, design of various commercial media for industrial fermentations – medium optimization methods

UNIT III STERILIZATION KINETICS

Thermal death kinetics of microorganisms, batch and continuous heat sterilization of liquid media, filter sterilization of liquid media, air sterilization and design of sterilization equipment – batch and continuous.

UNIT IV METABOLIC STOICHIOMETRY AND ENERGETICS

Stoichiometry of cell growth and product formation, elemental balances, degrees of reduction of substrate and biomass, available electron balances, yield coefficients of biomass and product
formation, maintenance coefficients energetic analysis of microbial growth and product formation, oxygen consumption and heat evolution in aerobic cultures, thermodynamic efficiency of growth.

UNIT V KINETICS OF MICROBIAL GROWTH AND PRODUCT FORMATION

Batch cultivation and continuous cultivation. Simple unstructured models for microbial growth, Monod model, growth of filamentous organisms, product formation kinetics – Leudeking- Piret models, substrate and product inhibition on cell growth and product formation. Biomass estimation – Direct and Indirect methods.

TOTAL: 45 PERIODS

COURSE OUTCOMES:

Upon completion of this course the student will be able to
 Identify suitable process instrumentation for monitoring and control of fermentor.
 Formulate the fermentation medium to facilitate improved product production.
 Select and apply the sterilization techniques in bioprocessing.
 Interpret the metabolic stoichiometry in microbial processes.
 Analyze the kinetics of microorganisms during fermentation processes.
 Develop strategies to solve the issues in bioprocessing.

TEXT BOOKS:

1. Peter F. Stanbury, Stephen J. Hall & A. Whitaker, Principles of Fermentation Technology, Butterworth-Heinemann; 3rd Edition ,2016.
2. Pauline M. Doran, Bioprocess Engineering Principles, Elsevier, 2nd Edition,2012

REFERENCES:

1. Michael L. Shuler, Fikret Kargi, Matthew De Lisa , Bioprocess Engineering , Prentice Hall, 3rd Edition, 2017
2. Bailey, James E. and David F. Ollis, “ Biochemical Engineering Fundamentals”, 2ndEdition. McGraw Hill , 1986.
3. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, CRC Press; 2nd Edition 1997