EL3304 Instrumental Methods of Analysis Syllabus:
EL3304 Instrumental Methods of Analysis Syllabus – Anna University Regulation 2021
OBJECTIVES:
• To impart knowledge on the principle and importance of various analytical instruments used for the characterization of various materials
UNIT I INTRODUCTION TO SPECTROSCOPICAL METHODS OF ANALYSIS
Electromagnetic radiation: various ranges, dual properties, various energy levels, interaction of photons with matter, absorbance & transmittance and their relationship, permitted energy levels for the electrons of an atom and simple molecules, various electronic transitions in organic and inorganic compounds effected by UV, and visible radiations, various energy level diagrams of saturated, unsaturated and carbonyl compounds, excitation by UV and visible radiations, choice of solvents, cut off wavelengths for solvents
UNIT II QUALITATIVE ANALYSIS BY UV AND VISIBLE SPECTROCOPY
Lamda max and epsilon max rules, Woodward -Fieser rules for the calculation of absorption maxima (Lamda max) for dienes and carbonyl compounds, Effects of auxochromes and effects of conjugation on the absorption maxima, Different shifts of absorption peaks(Bathochromic, hypsochromic, hypochromic), Instrumentation for UV and Visible spectrophotometers (source, optical parts and detectors), Applications of UV and Visible spectroscopy.
UNIT III QUANTITATIVEANALYSIS BY UV AND VISIBLE SPECTROCOPY
Beer-Lambert’s law, limitations, deviations (real, chemical, instrumental), estimation of inorganic ions such as Fe, Ni and estimation of nitrite using Beer -Lambert’s law, multicomponent analysis (no overlap, single way overlap and two way overlap), photometric titration(experimental set -up and various types of titrations and their corresponding curves).
UNIT IV IR SPECTROSCOPY
Theory of IR spectroscopy, various stretching and vibration modes for diatomic and triatomic molecules (both linear and nonlinear), various ranges of IR (near, mid, finger print and far) and their usefulness, Instrumentation (only the sources and detectors used in different regions), sample preparation techniques, qualitative analysis of alkanes, alkenes and carbonyl compounds.
UNIT V CHROMATOGRAPHIC METHODS
Classification of chromatographic methods, column, thin layer, paper, gas, High Performance Liquid Chromatographical methods (principle, mode of separation and technique).
TOTAL: 45 PERIODS
OUTCOMES:
At the end of this course, the students would
• Understand the fundamentals, concepts and mechanisms involved in spectral analysis.
• Understand the purpose and theories in qualitative analysis
• Understand the purpose and theories in quantitative analysis
• Understand the purpose and theories in IR spectral analysis
• Understand the purpose and theories of chromatographic methods
TEXT BOOKS :
1. Sivasankar B., “Instrumental Methods of Analysis”, Oxford University Press, 2012.
2. William Kemp, Organic Spectroscopy, 3rd Edition, Palgrave publishers, 2007.
REFERENCES:
1. Douglas A. Skoog, F. James Holler, Stanley R. Crouch, Instrumental Analysis, CENGAGE Learning, India, 7th Edition, 2007.
2. Willard H.H, Merritt L.L, Dean J.A and Settle F.A, Instrumental method of analysis, 7th edition, Wadsworth Publishing Company, 1988.
3. Gurdeep R. Chatwal, Sharma K. Anand, Instrumental methods of Chemical Analysis, Himalaya Publishers, New Delhi, 2014
4. John R Dyer, Applications of Absorption Spectroscopy of Organic Compounds, Prentice-hall of India Pvt. Ltd., 2012
5. Robert M. Silverstein, Francis X. Webstrer, David Kiemle, David L. Bryce, Spectrometric Identification of Organic Compounds, Wiley, 8th Edition, 2010.