CH3015 Enzyme Immobilisation Technology Syllabus:
CH3015 Enzyme Immobilisation Technology Syllabus – Anna University Regulation 2021
COURSE OBJECTIVES:
To understand Enzymes, homogeneity, and heterogenicity
To understand structural, functional properties, and metabolic pathways
To learn immobilization procedures, and types.
To design enzyme reactors
UNIT I INTRODUCTION
Catalysis and biocatalysis, Enzyme classification and nomenclature, enzyme structure, functionality and relationship, enzyme activity, enzyme sources, synthesis, recovery and purification, enzymes as process catalysts.
UNIT II HOMOGENEOUS ENZYME KINETICS
Hypothesis of enzyme kinetics, rapid equilibrium and steady-state hypothesis, determination of kinetic parameters, various types of kinetic inhibition, reactions with more than one substrate, effect of environmental variables- pH, temperature, and ionic strength.
UNIT III BASICS OF IMMOBILISATION
Immobilisation – Functional properties, Classification of Immobilisation techniques – Adsorption, matrix entrapment, crosslinking, covalent binding- advantages & disadvantages of each method, selection and characterisation of matrices for immobilisation, effect of physico chemical parameters on immobilised enzymes.
UNIT IV HETEROGENEOUS ENZYME KINETICS
Mass transfer effects in heterogeneous biocatalysis, partition effects, Immobilised enzyme kinetics – external (film) diffusion, internal (pore) diffusional kinetics, Thiele modulus and Effectiveness factor. Effects of electrostatic potential of the micro environment.
UNIT V ENZYME REACTORS & APPLICATION OF IMMOBILISED ENZYMES
Design of reactors with immobilised enzymes, Design of advanced immobilized enzyme systems, Application of immobilised enzymes in food industry, textile industry, Pharmaceutical industry & in medicine, in the production of biofuels, detergent industry, production of various bio-products, as biosensors.
TOTAL :45 PERIODS
OUTCOMES:
On completion of the course, the students will be able to
CO1 – understand the basic knowledge on classification of enzymes and their nomenclature
CO2 – understand Enzymes, homogeneity, and heterogenicity
CO3 – understand structural, functional properties, and metabolic pathways of enzymes
CO4 – learn immobilization procedures, and their different types.
CO5 – knowledge on designing enzyme reactors.
TEXT BOOKS:
1. “Enzyme Technology” by M.F.Chaplin and C.Bucke, Cambridge University press, 1990. (Website for the book, www:lsbu.ac.uk/biology/enztech/)
2. “Biocatalysts and Enzyme Technology” by K. Buchholz,V. Kasche and U.T. Bornscheur, Wiley,2005
REFERENCE BOOKS:
1. “Enzyme Technology”, by Shanmugam,S. and Satish Kumar,T.,IK International Pvt. Ltd, New Delhi, 2008
3. Enzyme Biocatalysis: Principles and Applications’ by A.Illanes, Springer,2008