CH3014 Bioseparation and Downstream Processing Syllabus:
CH3014 Bioseparation and Downstream Processing Syllabus – Anna University Regulation 2021
COURSE OBJECTIVES:
To learn the fundamentals of bio separations
To design unit operations steps for various downstream purification steps
To gain knowledge on theory, design, and application of bioprocessing.
UNIT I INTRODUCTION
Introduction to By-products and Bioseparation: Range and characteristics of bio products, Characteristics of Fermentation Broth, Selection of unit operation with due consideration of the physical, chemical and biochemical aspects of biomolecules. Stages of Downstream Processing
UNIT II CENTRIFUGATION AND FILTRATION
Primary Separation: Removal of insoluble and Biomass (and particulate debris) separation techniques, Flocculation and sedimentation, Centrifugation-Ultracentrifugation, Gradient centrifugation, Filtration: Theory of Filtration, Pre-treatment of Fermentation Broth, Filter Media and Equipment, Conventional and Cross-flow Filtration, Continuous Filtration, Filter cake resistance, specific cake resistance, Washing and dewatering of filter cakes
UNIT III ABSORPTION
Gas Absorption: Solubility of gases in liquids, Effect of temperature and pressure on solubility, Ideal and Non-ideal solutions, Choice of solvent for gas absorption, absorption factor, stripping factor, minimum gas liq ratio, Single stage gas absorption Cross Current, Co- current, Countercurrent, Multistage Counter current Operation, Absorption with Chemical Reactions, Related problems
UNIT IV EXTRACTION
Liquid-Liquid Separation Process: Single Stage Operation, Equipments for liquid-liquid extraction. Types of extraction processes: Reactive extraction, Aqueous two-phase systems, Reverse micellar extraction, solid-liquid extraction, Supercritical fluid Extraction. Different types of extractors and designing of extractors. Distillation: Simple, Steam and Equilibrium distillation, Fractionation, Mccabe Thiele method, azotropes
UNIT V CHROMATOGRAPHY AND MEMBRANE SEPARATION
Theory of chromatography, Shape and yield of a chromatographic peak, Binary chromatography, Hydrodynamic chromatography. Membrane-based bioseparation – Classification of membrane processes, Ultrafiltration, Microfiltration, Dialysis, Liquid membrane processes, Membrane chromatography, Electrophoresis, Affinity ultrafiltration, Field-flow fractionation
TOTAL :45 PERIODS
OUTCOMES:
On completion of the course, the students will be able to
CO1- understand the basic concept of bioseparation processes
CO2- acquire knowledge on theory, design, and application of bioprocessing
CO3- to understand the basic concepts absorption and their problems in bioprocessing
CO4- gather knowledge on extraction of bioproducts using different methods
CO5- acquire knowledge on chromatography techniques and their analysis, membrane separation process
TEST BOOKS:
1. Treybal R.E. , Mass transfer operation, 3 Ed., McGraw Hill New York, 1980.
2. Roger G. Harrison, Paul Todd, ScottR. Rudge, Demetri P. Petrides, Bioseparations Science and Engineering, Oxford University Press
3. B.Shivshankar, Bioseparations: Principles and Techniques, Eastern Economy Edition, PHI Learning Pvt. Ltd., Publishing House, New Delhi, 2012
4. Bioseparation & bioprocessing (2nd Ed) 2-Volume set, Ed SUBRAMANIAN Ganapathy, Wiley-VCH, ( 09-2007)
REFERENCES:
1. P.A. Belter, E.L. Cussler and Wei-Shou Hu., Bioseparations-Downstream Processing for Biotechnology, Wiley Interscience Publication, 1988.
2. R. K. Scopes, Berlin, Protein Purification: Principles and Practice, Springer, 1982. Scopes Ak, Protein Purification, IRL Press, 1993
3. Biotechnology: Bioprocessing, Rhem and Reed, Vol. 3, 1993
4. Separation and purification techniques in biotechnology, Fredreich Dechow, 1989
5. T. Schepler et al, Biotreatment, Downstream Processing and Modeling (Advances in Biochemical Engineering /Biotechnology, Vol 56) by Springer Verlag