CBM362 Principles of Tissue Engineering Syllabus:
CBM362 Principles of Tissue Engineering Syllabus – Anna University Regulation 2021
COURSE OBJECTIVES:
To study the cell types and differentiation.
To study basics about stem cells and its applications
To understand the methods and design involved in tissue engineering
UNIT I INTRODUCTION TO CELL BIOLOGY
Cell types – Progenitor cells – Cell growth and differentiation – Cell culture: Expansion – Transfer – Storage and Characterization – Cell signalling molecules – Growth factors – Cell attachment: Differential cell adhesion, Receptor-ligand binding – Cell surface markers.
UNIT II FUNDAMENTALS OF TISSUE ENGINEERING
History and scope of tissue engineering – Tissue organization – Tissue types: Epithelial, Connective – Vascularity and angiogenesis – Wound healing – Extra Cellular Matrix: Matrix molecules and their ligands – Tissue culture – Materials in tissue engineering.
UNIT III STEM CELLS
Definition of stem cells – Types of stem cells – Differentiation, dedifferentiation maturation, proliferation, pleuripotency and immortalization – Sources of stem cells: Haematopoetic – Fetal – cord blood – Placenta – Bone marrow – Primordial germ cells – Cancer stem cells – Induced pleuripotent stem cells.
UNIT IV ENGINEERING METHODS AND DESIGN
Soft lithography – Self-assembled monolayer, Micro contact printing, Micro fluidic patterning – Laminar flow patterning – Cell interaction with Polymer scaffolds and gels – Polymer scaffolds fabrications: Electro spinning – Solvent casting and particulate leaching – Micro fabrication of cell seeded scaffolds.
UNIT V APPLICATION OF TISSUE ENGINEERING
Replacement Engineering: Bone, cartilage, skin, blood, pancreas, kidney, heart valve and liver – Regenerative engineering: Peripheral Nerve regeneration, Cardiac tissue regeneration, Muscle regeneration – Regulation, Commercialization and Patenting.
COURSE OUTCOMES:
At the end of the course, the student should be able to:
CO1: Understand the basic concepts of tissue engineering
CO2: Acquire ability to function on multi-disciplinary teams
CO3: Apply the knowledge of professional and ethical responsibility in use of stem cells and gene therapy in creating tissue engineered therapies
CO4: Design and develop different biomaterial in tissue engineering application
CO5: Gain knowledge in research or clinical application on tissue repair/ engineering
TOTAL :45 PERIODS
TEXT BOOK
1. Robert P lanza, Robert Langer, Joseph Vacanti, “Principles of Tissue Engineering”, Academic Press, United States, 2020.
2. Donglu Shi, Qing Liu, “Tissue Engineering and Nanotheranostics”, World Scientific Publications, Singapore, 2018.
REFERENCES
1. Gary E. Wnek, Gary L Browlin, “Encyclopedia of Biomaterials and Biomedical Engineering”, Marcel Dekker Inc, New York, 2008.
2. R. Lanza, Anthony Atala (Eds), “Essential of Stem Cell Biology”, Academic Press, USA, 2013.
3. R. Lanza, Anthony Atala,“Handbook of Stem Cells’’, Academic Press, USA, 2012.