BT3461 Analytical Instrumentation Laboratory Syllabus:

BT3461 Analytical Instrumentation Laboratory Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES:

To train the students
• To have a practical hands on experience on Absoprtion Spectroscopic methods
• To acquire experience in the purification by performing chromatography
• To validate and analysis using spectrometric and microscopic techniques

EXPERIMENTS

1. Precision and validity in an experiment using absorption spectroscopy .
2. Validating Lambert-Beer’s law using KMnO4
3. Finding the molar absorbtivity and stoichiometry of the Fe (1,10 phenanthroline)3 using absorption spectrometry.
4. Finding the pKa of 4-nitrophenol using absorption spectroscopy.
5. UV spectra of nucleic acids.
6. Chemical actinometry using potassium ferrioxolate.
7. Estimation of SO4– by nephelometry.
8. Estimation of Al3+ by Flourimetry.
9. Limits of detection using aluminium alizarin complex.
10. Chromatography analysis using TLC.
11. Chromatography analysis using column chromatography.

TOTAL: 45 PERIODS

COURSE OUTCOME:

• The students would visualize and interpret the theory of spectroscopic methods by practice.

REFERENCES:

1. Skoog, D.A. etal. “Principles of Instrumental Analysis”, Vth Edition, Thomson / Brooks – Cole,1998.
2. Braun, R.D. “Introduction to Instrumental Analysis”, Pharma Book Syndicate, 1987.
3. Willard, H.H. etal. “Instrumental Methods of Analysis”, VIth Edition, CBS, 1986.
4. Ewing,G.W. “Instrumental Methods of Chemical Analysis”, Vth Edition, McGraw-Hill, 1985.