EN3302 Environmental Microbiology Syllabus:

EN3302 Environmental Microbiology Syllabus – Anna University Regulation 2021

COURSE OBJECTIVES:

 The course provides a basic understanding on microbiology relevant to environmental engineering for candidates with little prior knowledge of the subject
 The morphology, behaviour and biochemistry of bacteria, fungi, protozoa, viruses, and algae are outlined.
 The microbiology of wastewater, sewage sludge and solid waste treatment processes is also provided. Aspects on nutrient removal and the transmission of disease causing organisms are also covered.
 An exposure to toxicology due to industrial products and by-products are also covered.

UNIT I FUNDAMENTALS OF MICROBIOLOGY

Classification of microorganisms – prokaryotic, eukaryotic, cell structure, characteristics, importance, introduction to water, soil and air borne pathogens and Parasites and their effects on human, animal and plant health, transmission of pathogens, transmissible diseases – bacterial, viral, protozoan, and helminths parasites, concentration and detection of virus. control of microorganisms preservation of microorganisms, DNA, RNA, replication, recombinant DNA technology, their potential applications and intellectual property rights.

UNIT II MICROBIAL DIVERSITY AND NUTRIENT TURNOVER

Distribution of microorganisms in different environments – diversity of microorganisms – fresh and marine, terrestrial – microbes in surface soil, air – outdoor and Indoor, aerosols, bio safety in laboratory – extreme environment – archae bacteria – occurrence in water supplies – problems and control. biogeochemical cycles-nitrogen, carbon, phosphorus, sulphur – Role of Microorganism in nutrient cycle.

UNIT III METABOLISM OF MICROORGANISMS

Nutrition and metabolism in microorganisms, growth phases, carbohydrate, protein, lipid metabolism – respiration, aerobic and anaerobic-fermentation, glycolysis, Kreb’s cycle, hexose monophosphate pathway, electron transport system, oxidative phosphorylation, environmental factors, enzymes, bioenergetics, disruption in metabolism and disease. biodegradation of organic pollutants

UNIT IV MICROBIOLOGY OF WASTEWATER TREATMENT SYSTEMS

Microbiology of biological treatment processes – aerobic and anaerobic, α-oxidation, β oxidation, nitrification and denitrification, eutrophication. nutrients removal – BOD, nitrogen, phosphate. microbiology of sewage sludge – indicator organisms of water – coliforms – total coliforms, E-coli, streptococcus, clostridium, Bioleaching

UNIT V TOXICOLOGY

Ecotoxicology – toxicants and toxicity, factors influencing toxicity.effects – acute, chronic, test organisms – toxicity testing-lab and field testing methods, bioconcentration – Bioaccumulation, biomagnification, bioassay, biomonitoring.

TOTAL : 45 PERIODS
COURSE OUTCOMES:

On completion of the course, the student is expected to
CO1 Explain the basic importance and functional elements of environmental microbiology including the potential applications in the environment and intellectual property rights.
CO2 Understand and describe the type of microorganisms in the environment, their importance in water supplies and the role of microorganisms in the cycling of nutrients in an ecosystem.
CO3 Understand the metabolic processes on carbohydrates, protein and lipids, importance of enzymes, production of energy and the various additional metabolic processes.
CO4 Select and apply appropriate methods for assessing the water, air and soil borne pathogens, their health implications, importance of microbes in aerobic and anaerobic cycles and deterioration of water bodies.
CO5 Conduct testing and research on toxicology, understand the importance of test organisms, environmental applications such as biomagnifications, biomonitoring and in developing risk based standards.

REFERENCES:

1. Bhatia S.C. , “Hand Book of Environmental Microbiology”, Part 1 and 2, Atlantic Publisher, 2008
2. Gabriel Bitton, Wastewater Microbiology, 3rd Edition, 2005
3. Raina M. Maier, Ian L. Pepper, Charles P. Gerba, “Environmental Microbiology”, Academic Press, 3rd Edition 2014
4. Volodymyr Ivanov,Environmental Microbiology for Engineers 2nd Edition, CRC Press, 2021, ISBN 9781498702126
5. N duka Okafor, Environmental Microbiology of Aquatic and Waste systems. Springer Publishers, 2011, ISBN 978-94-007-1459-5
6. Stanley E. Manahan, “Environmental Science and Technology”, Lewis Publishers, 2008.
7. Hurst, C.J. Manual of “Environmental Microbiology”. 2nd Ed. ASM PRESS, Washington, D.C. ISBN 1-55581 – 199 – X. 2007
8. Frank C. Lu and Sam Kacew, LU‟s Basic Toxicology, Taylor & Francis, London 5th Ed, 2009.