CIC340 Analytical Instrumentation Syllabus:
CIC340 Analytical Instrumentation Syllabus – Anna University Regulation 2021
COURSE OBJECTIVES:
1. To understand the theory and operational principles of instrumental methods for identification and quantitative analysis of chemical substances by different types of spectroscopy.
2. To impart fundamental knowledge on gas chromatography and liquid chromatography.
3. To integrate a fundamental understanding of the underlining principles of physics as they relate to specific instrumentation used for gas analyzers and pollution monitoring instruments.
4. To impart knowledge on the important measurement in many chemical processes and laboratories handling liquids or solutions.
5. To understand the working principle, types and applications of NMR and Mass spectroscopy
UNIT I SPECTROPHOTOMETRY
Spectral methods of analysis – Beer-Lambert law – UV-Visible spectroscopy – IR Spectrophotometry – FTIR spectrophotometry – Atomic absorption spectrophotometry – Flame emission and atomic emission photometry – Construction, working principle, sources detectors and applications.
UNIT II CHROMATOGRAPHY
General principles – classification – chromatographic behaviour of solutes – quantitative determination – Gas chromatography – Liquid chromatography – High-pressure liquid chromatography – Applications.
UNIT III INDUSTRIAL GAS ANALYZERS AND POLLUTION MONITORING INSTRUMENTS
Gas analyzers – Oxygen, NO2 and H2S types, IR analyzers, thermal conductivity detectors, analysis based on ionization of gases. Air pollution due to carbon monoxide, hydrocarbons, nitrogen oxides, sulphur dioxide estimation – Dust and smoke measurements
UNIT IV pH METERS AND DISSOLVED COMPONENT ANALYZERS
Selective ion electrodes – Principle of pH and conductivity measurement – dissolved oxygen analyzer – Sodium analyzer – Silicon analyzer – Water quality Analyzers.
UNIT V NUCLEAR MAGNETIC RESONANCE AND MASS SPECTROMETRY
NMR – Basic principles – Continuous and Pulsed Fourier Transform NMR spectrometer – Mass Spectrometry – Sample system – Ionization methods – Mass analyzers – Types of mass spectrometry.
TOTAL : 45 PERIODS
SKILL DEVELOPMENT ACTIVITIES (Group Seminar/Mini Project/Assignment/Content Preparation / Quiz/ Surprise Test / Solving GATE questions/ etc)
1. A seminar on applications of various analytical techniques.
2. Selection of Chromatography for applications.
3. Familiarization of any one relevant software tool (MATLAB/ SCILAB/ LABVIEW/ Proteus/Equivalent open source software)
4. Realization of spectroscopy and analyzers in hardware
5. Introduction to other advanced spectroscopy and analyzer not covered in the above syllabus
COURSE OUTCOMES:
CO1 Understand the basic concept of qualitative and quantitative analysis of a given sample.
CO2 Explain the working knowledge of analytical instrumentation typically employed in chemical/biochemical research and industry laboratories.
CO3 Apply the fundamental principles of selective analytical instruments for separation, identification and quantitative analysis of chemical substances.
CO4 CO4Differentiate between online and offline process and identify suitable instruments for analysis.
CO5 Describe the relative strengths and limitations of different instrumental based analysis methods.
CO6 Identify and suggest a suitable analytical method for a specific application.
TEXT BOOKS:
1. Braun, R.D., “Introduction to Instrumental Analysis”, Pharma Book Syndicate, Singapore, 2nd edition 2016.
2. Willard, H.H., Merritt, L.L., Dean, J.A., Settle, F.A.,”Instrumental methods of analysis”, CBS publishing & distribution, 7th Edition, 2012.
3. Robert E. Sherman., “Analytical Instrumentation, Instruments”, Society of America, 1996.
REFERENCES:
1. Khandpur, R.S., “Handbook of Analytical Instruments”, Tata McGraw Hill publishing Co. Ltd., 5th edition 2018.
2. Ewing, G.W., “Instrumental Methods of Chemical Analysis”, McGraw Hill, 5th edition reprint 1985. Digitized in May 2013.
3. Liptak, B.G., “Process Measurement and Analysis”, CRC Press, 5th Edition, 2016.
4. NPTEL lecture notes on, “Modern Instrumental methods of Analysis” by Dr.J.R. Mudakavi, IISC, Bangalore